BS-2081F (LED) Trinocular LED Research Fluorescent Biological Microscope
BS-2081F(LED)
Introduction
BestScope continues to explore the research needs of specialized fields such as pathology, cytology and virology, and continuously optimizes and upgrades the BS-2081 series of scientific grade upright microscopes to have near-perfect optical performance and mechanical structure design. NIS Infinity Optical System has precise imaging and chromatic aberration correction capabilities. The illumination system with high color reproduction, high-quality optics and full-featured accessories makes these microscopes ideal for cutting-edge life science research where darkfield of view, differential interference contrast or high-performance fluorescence are required. In addition, a wide range of motorized and intelligent components and powerful imaging software meet the needs of quick sample overview and detailed sample inspection, making repetitive tasks easy and greatly increasing ease and comfort for maximum productivity. No matter in a clinical laboratory or a research laboratory, the BS-2081 series microscopes provide the ideal microscopy imaging solution.
Feature
Sapphire Glass Stage is optional.
Mechanical stage with sapphire glass insert is optional, it is endurable, never could be scratched and allows users to clear the stage easily.
Put Slide by One Hand.
It is easy for users to put slides by one hand due to the special designed slide clip.
Tilting Trinocular Head is optional.
(1) The eye tube can be adjusted from 0°-35°.
(2) Digital cameras or DSLR cameras can be connected to the trinocular tube.
(3) The beam splitter has 3-position (100:0, 20:80, 0:100).
The splitter bar can be assembled on the either side according to user’s requirements.
ECO Function
The transmitted light would be off automatically after 30 minutes from operators leave. It can not only save energy, but also keep the lamp life longer.
Low Position X-Y Knobs.
The height of the stage control knobs can be adjusted up or down by 18mm to ensure a comfortable hand position, the tension of X-Y control knob also can be adjusted.
BS-2081L has Intelligent operating system.
(1) Coded Nosepiece with light intensity memory function.
The BS-2081L microscope can memorize the illumination brightness when using each objective. When the objective has been changed, the light intensity will be automatically adjusted to reduce visual fatigue and improve work efficiency.
(2) Use a dimming knob to achieve multiple functions.
One Click: Enter standby status
Double Clicks: Light lock or unlock
Rotation: Adjust brightness
Press + Up-spin: Switch to the upper light source
Press + Down-spin: Switch to the under light source
Press 3 seconds: Set the time of turning off the light after leaving.
(3) The display of microscope working status.
The LCD on the front of the microscope can display the working status of the microscope, including magnification, light intensity, sleepy model and so on.
(4) Color temperature adjustment function.
The color temperature can be adjusted from yellow to white, which can lead to a better contrast and meet the requirements of various research.
Application
BS-2081 series research microscopes are ideal instrument in biological, histological, pathological, bacteriology, immunizations and pharmacy field and can be widely used in medical and sanitary establishments, laboratories, institutes, academic laboratories, colleges and universities.
Specification
Item |
Specification |
BS-2081 |
BS-2081L |
BS-2081F |
BS-2081F (LED) |
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Optical System | NIS60 Infinite Color Corrected Optical System |
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Viewing Head | Seidentopf Trinocular Head, 30° inclined, interpupillary distance: 47mm-78mm; splitting ratio Eyepiece: Trinocular=100:0 or 20:80 or 0:100 |
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Ergo Tilting Trinocular Head, adjustable 0-35° inclined, interpupillary distance 47mm-78mm; splitting ratio Eyepiece: Trinocular=100:0 or 20:80 or 0:100 |
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Seidentopf Binocular Head, 30° inclined, interpupillary distance: 47mm-78mm |
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Eyepiece | Super wide field plan eyepiece SW10X/25mm, diopter adjustable |
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Super wide field plan eyepiece SW10X/22mm, diopter adjustable |
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Extra wide field plan eyepiece EW12.5X/17.5mm, diopter adjustable |
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Wide field plan eyepiece WF15X/16mm, diopter adjustable |
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Wide field plan eyepiece WF20X/12mm, diopter adjustable |
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Objective | N-PLN Plan Objective | N-PLN 2X/NA=0.06, WD=7.5mm |
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N-PLN 4X/NA=0.10, WD=30mm |
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N-PLN 10X/NA=0.25, WD=10.2mm |
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N-PLN 20X/NA=0.40, WD=12mm |
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N-PLN 40X/NA=0.65, WD=0.7mm |
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N-PLN 100X(Oil)/NA=1.25, WD=0.2mm |
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N-PLN 50X(Oil)/NA=0.95, WD=0.19mm |
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N-PLN 60X/NA=0.80, WD=0.3mm |
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N-PLN-I 100X (Oil, with Iris Diaphragm)/ NA=0.5-1.25, WD=0.2mm |
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N-PLN PH Plan Phase Contrast Objective | N-PLN PH 10X/NA=0.25, WD=10.2mm |
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N-PLN PH 20X/NA=0.40, WD=12mm |
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N-PLN PH 40X/NA=0.65, WD=0.7mm |
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N-PLN PH 100X(Oil)/NA=1.25, WD=0.2mm |
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N-PLFN Plan Semi-apochromatic Fluorescent Objective | N-PLFN 4X/NA=0.13, WD=17.2mm |
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N-PLFN 10X/NA=0.30, WD=16.0mm |
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N-PLFN 20X/NA=0.50, WD=2.1mm |
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N-PLFN 40X/NA=0.75, WD=1.5mm |
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N-PLFN 100X(Oil)/NA=1.4, WD=0.16mm |
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N-PLFN PH Plan Semi-apochromatic Fluorescent Phase Contrast Objective | N-PLFN PH 10X/NA=0.30, WD=15.8mm |
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N-PLFN PH 20X/NA=0.50, WD=2.7mm |
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N-PLFN PH 40X/NA=0.75, WD=1.35mm |
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N-PLFN PH 100X(Oil)/NA=1.40, WD=0.18mm |
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N-PLPN Plan Apochromatic Objective | N-PLPN 10X/NA=0.45, WD=4.0mm |
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N-PLPN 20X/NA=0.75, WD=1.1mm |
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N-PLPN 40X/NA=0.95, WD=0.21mm |
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N-PLPN 60X(Oil)/NA=1.42, WD=0.25mm |
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N-PLPN 100X(Oil)/NA=1.45, WD=0.13mm |
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Nosepiece | Backward Sextuple Nosepiece (with DIC slot) |
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Backward Coded Sextuple Nosepiece (with DIC slot) |
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Condenser | Swing-out type condenser N.A.0.9/0.25 |
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Turret Phase Contrast Condenser |
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Dark-field Condenser (Dry), used for objectives lower than 100X |
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Dark-field Condenser (Oil), used for 100X objective |
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Transmitted Illumination | 3W S-LED lamp, center pre-set, intensity adjustable |
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3W S-LED lamp, center pre-set, intensity adjustable. LCD screen with illumination management system |
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12V/100W halogen lamp, center pre-set, intensity adjustable |
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Focusing | Low-position coaxial coarse and fine focusing, fine division 1μm, Moving range 35mm |
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Stage | Double layers mechanical stage, size 190mmX152mm; moving range 78mmX54mm (double slide holder, Right or left handle); precision: 0.1mm, with Ultra-hard Glass Insert |
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Double layers mechanical stage, size 190mmX152mm; moving range 78mmX54mm (double slide holder, Right or left handle); precision: 0.1mm; with Sapphire Crystal Glass Insert |
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DIC Kit | Polarizer for DIC Kit |
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DIC insert plate(10X/20X), can be inserted into the DIC slot on nosepiece |
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DIC insert plate(40X/100X) can be inserted into the DIC slot on nosepiece |
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DIC Turret Condenser (10X, 20X/40X, 100X Warrior Prism inside) |
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Reflected Fluorescence Illuminator (with mercury lamp) | Turret with 6 filter block cubes position, with iris field diaphragm and aperture diaphragm, central adjustable; with filter slot; with fluorescence B, G filters |
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100W mercury lamp house, filament center and focus adjustable; with reflected mirror, mirror center and focus adjustable. |
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Digital power controller, wide voltage 100-240VAC |
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ND6/ND25 Filter |
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U, V, R, FITC, DAPI, TRITC, Auramine, mCherry, FL-BG fluorescent filters |
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Reflected Fluorescent Attachment (with LED lamps) | LED Reflected Fluorescent Attachment, Turret with 6-position for filter block cubes, including B, G fluorescent filters and B, G, U, R LED lamps (the LED lamps can be used for B, G, U, R, FITC, DAPI, TRITC fluorescent filters), there are 4 positions for the LED lamps |
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U, R, FITC, DAPI, TRITC fluorescent filters |
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Filter for Transmitted Illumination | Green Filter 45mm |
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Blue Filter 45mm |
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Yellow Filter 45mm |
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Red Filter 45mm |
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ND6 Filter |
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ND25 Filter |
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Other Accessories | 0.5X C-mount Adapter |
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1X C-mount Adapter |
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Dust Cover |
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Power Cord |
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Cedar Oil 5ml |
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Simple Polarizing kit |
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Calibration slide 0.01mm |
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Multi Viewing Attachment for 2/3/5/7/10 person |
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Note: ● Standard Outfit, ○ Optional
Accessories
- N-PLN Series Plan Objectives.
- The plan objectives can provide flat high transmittance image from visible light to NIR light. They are usually used for bright-field viewing as the high signal-to-noise, high resolution and high contrast features.
- N-PLN PH Series Plan Phase Contrast Objectives.
- These plan phase contrast objectives are specially designed for phase contrast observation. They are good choice for clinic and scientific research. These objectives can provide advanced flat image of 25mm FOV under transmitted bright field.
- N-PLFN Series Plan Semi-APO Fluorescent Objectives.
- Owe to the multilayers coating technology, these Semi-APO objectives can compensate the spherical aberration and the chromatic aberration from ultraviolet and infrared light. High-sensitive fluorescence performance of the objectives ensures the sharpness, definition and color rendition of images.
N-PLPN Plan Apochromatic Fluorescent Objective
The newly developed advanced apochromatic objective lenses have high level of chromatic aberration correction capability and high resolution, ensuring a high level of wave-phase contrast correction with full field of view, these APO objectives are ideal for routine laboratory observation work and digital imaging.
Mammary Gland (active stage)
Bright field Viewing.
Brighter image, high resolution and flatness, suitable for all the magnifications.
Arterial Cell
Fluorescent Viewing.
The compact epi-fluorescent components include noise elimination feature which ensures images captured are bright, with high contrast and high signal-to-noise ratio.
Excitation |
Excitation Filter |
Dichroic Mirror |
Barrier Filter |
LED lamp Wave Length |
Application |
B |
BP460-495 |
DM505 |
BA510 |
485nm |
·FITC: Fluorescent antibody method·Acidine orange: DNA, RNA
·Auramine: Tubercle bacillus ·EGFP, S657, RSGFP |
G |
BP510-550 |
DM570 |
BA575 |
535nm |
·Rhodamine, TRITC: Fluorescent antibody method·Propidium iodide: DNA
·RFP |
U |
BP330-385 |
DM410 |
BA420 |
365nm |
·Auto-fluorescence observation·DAPI: DNA staining
·Hoechest 332528, 33342: used for Chromosome staining |
V |
BP400-410 |
DM455 |
BA460 |
405nm |
·Catecholamines·5-hydroxy tryptamine
·Tetracycline: Skeleton, Teeth |
R |
BP620-650 |
DM660 |
BA670-750 |
640nm |
·Cy5·Alexa Fluor 633, Alexa Fluor 647 |
FITC |
BP460-495 |
DM505 |
BA510-550 |
485nm |
·FITC: Fluorescent antibody method |
DAPI |
BP360-390 |
DM415 |
BA435-485 |
365nm |
DAPI: DNA staining |
TRITC |
BP528-553 |
DM565 |
BA578-633 |
535nm |
·TRITC: Fluorescent antibody method |
FL-Auramine |
BP470 |
DM480 |
BA485 |
450nm |
·Auramine O, Testing for tuberculosis |
Texas Red |
BP540-580 |
DM595 |
BA600-660 |
560nm |
It is commonly conjugated to antibodies and proteins for cellular imaging applications. |
mCherry |
BP542-582 |
DM593 |
BA605-675 |
560nm |
Molecular labeling and cellular component localization |
FL-BG |
BP 453-490/533-588 |
DM 495-548/595-705 |
BA 500-540/603-664 |
485nm/525nm |
·FITC: Fluorescent antibody method·Acidine orange: DNA, RNA
·Auramine: Tubercle bacillus ·EGFP, S657, RSGFP ·Rhodamine, TRITC: Fluorescent antibody method ·Propidium iodide: DNA ·RFP |
Note:
1. All the above fluorescent filters can be used on BS-2081F.
2. Filters Size in fluorescent block: Excitation and Barrier Filters is Φ24.8mmX3.6mm (thickness with metal edge), Dichroic Mirror is 37.5X28.5X1mm, the filters can be installed in the block.
Rat Ovarian Cell
Phase Contrast Viewing.
Users can get high contrast image of neutral background color whatever the magnification is. It is suitable for viewing non-stained specimen.
Uric Acid Crystal
Polarizing Viewing.
It is quite suitable for viewing collagen, amyloid and crystal etc., double refracting specimens.
Spirogyra
Dark-field Viewing.
It can be used for clearly viewing of blood or flagellum etc., fine structure.
DIC Attachment.
The DIC attachment can be used to observe unstained specimens. It including:
1. Semi-APO objective
2. Turret DIC condenser
3. Polarizer
4. DIC Plate
Multi Viewing Heads
BS-2083MH4A (For 2 users, Face to Face)
BS-2083MH4B (For 2 users, Side by Side)
BS-2083MH6(For 3 users)
BS-2083MH10(For 5 users)
BS-2081MH20 (For 10 users)
Note: We have not captured images with BS-2081, so we use BS-2083 to illustrate, BS-2081MH series are different with BS-2083 series on main microscope body.
LED Fluorescent Attachment.
The LED Reflected Fluorescent Attachment has turret with 6-position for filter block cubes, standard configuration including B, G fluorescent filters and B, G, U, R LED lamps, the LED lamps can be used for B, G, U, R, FITC, DAPI, TRITC fluorescent filters (U, R, FITC, DAPI, TRITC fluorescent filters are optional), there are 4 positions for the LED lamps. The fluorescent filters are reliable and precise, which offers high performance for various demands.
Sample Images
System Diagram
Dimension
BS-2081&BS-2081L
BS-2081F
Unit: mm
Certificate
Logistics