BCF295 Laser Scanning Confocal Microscopy

Item

Specification

BCF295

Optical system

NIS60 Infinite optical system(F200)

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Laser

Laser 405 nm, 488 nm, 561 nm, 640 nm

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Detector

Wavelength: 400-750nm, detector: 3 PMT (photomultiplier tube)

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Scanning head

Maximum pixel size: 2048 x 2048

Scanning speed: 2 fps (512 x 512 pixels, bidirectional), 18 fps (512 x 32 pixels, bidirectional)

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Pinhole

Round, 6 sizes

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Confocal field of view

φ20mm inscribed square

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Software

2D display/image processing/analysis

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Eyepiece (field of view)

10×/25mm, EP17.5mm, adjustable diopter -5～+5, interface Φ30mm

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Viewing Head

Siedentopf trinocular viewing head, inclined at 45°, Interpupillary 47-78mm, eyepiece interface Φ30mm, fixed diopter; Eyepiece/camera switching: (100/0,50/50,0/100); eyepiece/close eyepiece/adjustable Bertrand lens

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NIS60 Objective

10× Apochromatic objective, NA=0.45, WD=4.0mm, Cover slip=0.17mm

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20× Apochromatic objective, NA=0.75, WD=1.1mm, Cover slip=0.17mm

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40× Apochromatic objective, NA=0.95, WD=0.21mm, Cover slip=0.11-0.23mm

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60× Apochromatic objective, NA=1.42, WD=0.25mm, Cover slip=0.17mm (Oil)

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100× apochromatic objective, NA=1.45, WD=0.13, Cover glass=0.17 (Oil)

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Nosepiece

Motorized sextuple nosepiece (with expansion slot), M25×0.75

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Stage

Motorized control (conventional type): moving range 130 mm x 100 mm (stage size 325 mm x 144 mm), Maximum speed: 50mm/s; resolution: 0.1μm, repeat accuracy: ±1μm (common type 2.5um), absolute accuracy: ±5μmIt, can be equipped with three special sample holder adapters such as multi-well plate, 35mm culture dish and slide plate

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Condenser

6-hole electric control: NA0.55, WD26; phase contrast (10/20, 40, 60(optional)), DIC (10X, 20X/40X), Empty hole is optional

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Focusing system

Coaxial coarse and fine focusing mechanism, stroke: 7mm up and 2mm down; coarse adjustment 2mm/rotation, fine adjustment 0.002mm/rotation; manual and electric control, minimum step 0.02um when electric control

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Illumination System

Transmitted Kohler illumination, 10W LED

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Epi-illumination: 100W mercury lamp illumination; field of view/aperture diaphragm; 3-hole color filter insert (with ND6 and ND25 filters)

6-hole electric fluorescent turntable (standard for B, G, U); electric fluorescent shutter

Body port

Split ratio: Left: eyepiece = 100:0; right: eyepiece = 80: 20

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Intermediate magnification

Manual 1X, 1.5X switching

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DIC Plate

10X, 20X, 40X plug-in plate; can be placed in the converter slot

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Power control box

Can display objective magnification, fluorescence band, etc.

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Microscope shock-proof table and Computer

1. Microscope shock-proof table: air cushion type confocal special ≥1200mm×800mm shock-proof table, the panel adopts high permeability stainless steel plate.

2. Computer workstation: a set of HP workstations or similar performance workstations.

(1) HP Z840/CT Workstation/English OS Windows 7 64bit Professional Edition

(2) CPU: Intel Xeon E5-26434C 3.30 10MB 1600 x 1 or similar performance

(3) RAM: 32GB DDR -1600 ECC or similar performance

(4) HDD: 1TB 7200 RPM SATA 1ST HDD or similar performance

(5) 16X SuperMulti DVDRW SATA 1st ODD or similar performance

(6) Display: 2PCS ≥20 inch LED backlit widescreen IPS LCD displays.

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